What would Dr. James Parkinson think today? II. Neuroimaging in Parkinson’s disease

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/136297/1/mds26951.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/136297/2/mds26951_am.pd

Complementary motivational roles of nigroaccumbens and nigrostriatal dopaminergic pathways

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/147822/1/mds27504_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/147822/2/mds27504.pd

Many genes involved in Tourette syndrome pathogenesis

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/137748/1/mds27070.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/137748/2/mds27070_am.pd

Receptor Binding Techniques

This overview first discusses issues relating to the selection of radioligand for receptor binding assays, including the isotopic label and considerations pertaining to the pharmacological and chemical profile of the ligand. This is followed by a section on characterization of ligand‐binding assays, starting with tissue preparation methods, followed by detection of specific binding, determination of incubation and washing conditions and a discussion of saturation and competition assay formats. Quantification of the assay results can be accomplished by autoradiography or film densitometry. Finally, methods and considerations for analysis of the resulting data are presented.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/143702/1/cpns0104.pd

A Failed Future

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/156468/2/mds28130.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/156468/1/mds28130_am.pd

End of lines and boxes

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/34959/1/1086_ftp.pd

The pleiotropic gene theory of senescence: Supportive evidence from human genetic disease

Senescence is a universal but poorly understood phenomenon among metazoans. One theoretically convincing but unproven evolutionary theory of senescence is the pleitropic gene theory of Williams (1957). This paper develops the hypothesis that some human genetic diseases exemplify the type of phenotypic effects predicted by this theory. The evidence supporting this contention is reviewed and ways of testing this hypothesis are suggested. Other human genetic diseases could be examined in the same manner. Confirmation of this theory would have significant implications for the study of aging.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/27085/1/0000076.pd

Quantitative autoradiography of 4'-ethynyl-4-n-[2,3-3H2]propylbicycloorthobenzoate binding to the GABAA receptor complex

4'-Ethynyl-4-n-[2,3-3H2]propylbicycloorthobenzoate ([3H]EBOB) binding to the GABAA receptor complex was characterized autoradiographically in rat brain and then its binding in human brain was investigated. [3H]EBOB binding was saturable, specific and identified a single population of binding sites. The Kd obtained from saturation was 4.59 nM. Picrotoxin produced dose-dependent inhibition of [3H]EBOB binding and saturation analysis indicated a competetitive interaction. Isoguvacine inhibited [3H]EBOB binding with regionally different effects. Bicuculline increased [3H]EBOB binding only in the cerebellar granule cell layer. In human cerebellum, a high level of [3H]EBOB binding sites was seen in the granule cell layer. These results suggest that [3H]EBOB binds to the picrotoxin binding site associated with the GABAA receptor complex, that regional differences in GABAA agonist and antagonist modulation of [3H]EBOB binding reflect underlying regional differences in GABAA receptor subunit composition, and that there is a species difference in GABAA receptor distribution between human and rat cerebellum.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/31320/1/0000229.pd

GABAA, GABAB, and benzodiazepine binding sites in the cerebellar cortex of the red-eared turtle (Pseudemys scripta)

We used receptor autoradiography to ascertain the distribution of GABAA and GABAB binding sites in the cerebellar cortex of the red-eared turtle (Pseudemys scripta). GABAA binding sites were found in both molecular and granule cell layers with highest levels in the granule cell layer. GABAB binding sites were found at highest level in the molecular layer. Benzodiazepine binding sites were found in approximately equal abundance in both layers. Little binding of any ligand was seen in the Purkinje cell layer. Our results are similar to those found in mammals and other non-mammalian vertebrates and indicate that the organization of inhibitory pathways of the cerebellar cortex has been conserved in the course of vertebrate evolution.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/29731/1/0000067.pd

Parasagittal zonation of GABA-B receptors in molecular layer of rat cerebellum

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/27682/1/0000065.pd